Genome-Wide Characterization of NLRs in Saccharum spontaneum L. and Their Responses to Leaf Blight in Saccharum

Sugarcane is an important sugar and potential energy crop, the complexity of its genome has led to stagnant progress in decipherment hindered genome-wide analysis nucleotide binding site leucine-rich repeat (NLR) receptor until Saccharum spontaneum was published. From S. spontaneum, 724 allelic non-allelic NLRs were identified classified into five types (N, NL, CN, CNL, P) according domain architectures integrity at least 35 genes encoded non-canonical domains. The phylogenetic indicated containing coiled-coil (CC) separated from those without CC six Poaceae species, including spontaneum. motif determined characteristics functions 137 representative NLRs, especially core motifs contained NBS LRR domains, which that regularly distributed among clades. Through transcription factor (TFBS) profiles, we predicted most regulator sugarcane ERF, MIKC_MADS, C2H2. In addition, based on three sets transcriptome data two hybrids one clone infected by necrotrophic fungal pathogen Stagonospora tainanensis causing leaf blight (SLB), expression dynamics responding infection clones compared. different genetic background significant difference response SLB clones, got inference mechanism resistance. These results provided a basic reference new insights further study utilize NLRs.